Isolation and characterization of 15 microsatellite loci from mango (Mangifera indica L.) and cross-species amplification in closely related taxa.
Schnell R. J., Olano C. T., Quintanilla W. E., Meerow A. W.
Author Affiliation: USDA-ARS-National Germplasm Repository, 13601 Old Cutler Rd., Miami, FL 33158, USA.
Molecular Ecology Notes 5 : 625-627
Abstract : We report here on the development and characterization of 15 microsatellite loci isolated from Mangifera indica L. These markers were evaluated using 59 Florida cultivars and four related species from the USDA germplasm collection for mango. Two loci were monomorphic and 13 polymorphic, with two to seven alleles per locus. Four loci departed significantly from Hardy-Weinberg equilibrium and have significant heterozygote deficiency. Nine loci exhibited significant linkage disequilibrium. Cross-species amplification was successful in four related species. These loci are being used to investigate patterns of genetic variation within M. indica and between closely related species.