Characterization of mango germplasm using random amplified polymorphic DNA and morphological markers.
Weeraratne W. A. P. G., Samarajeewa P. K., Nilanthi R. M. R.
Author Affiliation: Field Crops Research and Development Institute, Maha Illuppallama, Sri Lanka.
Annals of the Sri Lanka Department of Agriculture 7 : 289-300
Abstract : Random amplified polymorphic DNA (RAPD) method was used to evaluate the genetic diversity among 11 cultivars of Mangifera indica and endemic species M. zeylanica. Ten random primers were used for RAPD analysis. Primer OPA-12 was the most useful for the characterization of mango germplasm. The lowest genetic distance was observed between Peterpasand and Vellai Colomban (0.100), whereas the greatest genetic distance was observed between Kohuamba and Polamba, Walamba and Willard, and M. zeylanica and Malwana (0.965). Discrepancies were evident between the results of analysis based on dendrograms obtained by RAPD analysis and those based on the analysis of morphological traits. The results revealed that the characterization of mango germplasm can be supplemented with analysis using molecular markers.