Characterization and genetic diversity of mango (Mangifera indica L.) in Nepal.
Subedi A., Bajracharya J., Joshi B. K., Regmi H. N., Gupta S. R., KC H. B.
: 167-175
Abstract : Mango (Mangifera indica L.) is an important tropical fruit of Nepal and occurs as a domesticated or wild entity in the complex biotic community of the ecosystem. Wide ecological variation and diverse needs of multi-ethnic communities have enriched the country with genetic wealth of diverse mango varieties as landraces and this diversity is considered a valuable economic asset for on-farm conservation in Nepal. Siraha, Saptari, Dhading, Kavre, Parbat, Baglung and Dadeldhura districts representing the tropical and subtropical ecosystems were surveyed to collect and characterize the diversity of mango. A total of 216 fruit samples and 23 young leaf samples of mango landraces were collected and evaluated for 19 qualitative and quantitative morphological and isozyme characteristics to compare the genetic variations and relationships. A range of variation existed in mango for fruit with different shapes, colour, sizes, flavour (aroma) and taste with 25-47 coefficients of variation and 0.592-0.865 Shannon Weaver index. Siraha and Dadeldhura are rich in mango diversity. Principal component analysis of qualitative traits and quantitative traits of fruits showed the existence of a continuum of mango diversity in Nepal with no ecogeographic differentiation. Qualitative traits were of importance in determining the groupings and relationships. Esterase isoenzyme profiles and cluster analysis based on allele frequencies revealed genetic variation (0.33-1.00 Jaccard's similarity coefficient) and showed relationships among farmers' varieties. The observed variation in morphological and esterase isoenzyme of mango is discussed in the context of on-farm conservation.