Multiplication of mango (Mangifera indica L.) cv. Sein-ta-lone through somatic embryogenesis.
Thida, Hla Aung, Khin Soe
Author Affiliation: Biotechnology Section, Department of Agricultural Research (DAR), Yezin, Myanmar.
Proceedings of the Annual Research Conference (Agricultural Sciences), Yangon, Myanmar, 7-9 January, 2005 : 157-177
Abstract : Nucellar tissues of Sein-ta-lone mango (Mangifera indica L.) were used to verify their response, in callus or somatic embryo induction, on different media. Nucellar tissues showed positive response to all of the induction media except the one containing no hormone and 20% coconut water. It was observed that somatic embryos were initiated at 21 days (earliest) and at 55 days (latest) after explant inoculation. On all media, nucellar explant survival rates (%) were not significantly different from one another at 4 weeks after culture (WAC) but they were different at 8WAC. Nucellar explants should be recommended for further study to obtain somatic embryogenesis in mango. This study also revealed that basal medium containing B5 macro salts, MS micro salts and vitamins supplemented with 2,4-D 0.5 mgl-1 (2.3 µM) and 20% coconut water was suitable for high somatic embryo induction percentage and the same basal medium with 2,4-D 2.0 mgl-1 (9µM) and 20% coconut water gave the earliest somatic embryo induction.