Somatic embryogenesis from nucellar tissues of mango (Mangifera indica Linn.).
Jisha Mathew, Jayaram K. M., Nabeesa Salim
Author Affiliation: Division of Plant Physiology and Biochemistry, Department of Botany, University of Calicut, Calicut - 673 635, Kerala, India.
Plant Cell Biotechnology and Molecular Biology 7 : 175-178
Abstract : Callus induction and somatic embryogenesis were obtained from de-embryonated nucellar tissues of young mango (Mangifera indica Linn.) cultivar 'Natumanga' fruits on MS medium supplemented with 4.52 µM 2,4-D. Mostly globular/heart shaped embryos from each half of the nucellus was noticed within a period of 80 days. Embryos transferred to MS medium with 2.26 µM 2,4-D showed secondary asynchronous embryogenesis resulting in globular, heart shaped and cotyledonary stages of development. Histological studies were conducted to confirm the developmental stages of embryos. Embryo germination was inhibited due to the abundance of phenolics oozed out to the medium.