Identification of molecular markers linked with differential flowering behaviour of mangoes in Andaman and Nicobar Islands.
Damodaran T., Medhi R. P., Dev G. K., Damodaran V., Rai R. B., Kavino M.
Author Affiliation: Central Agricultural Research Institute, Port Blair 744 101, India.
Current Science 92 : 1054-1056
Abstract : In a study conducted to find the cause of differential flowering in mangoes, approximately 30 clones comprising 20 single-flowering and 10 multiple-flowering types were selected. Among them, three multiple-flowering local types (GL 1, GL 2 and GL 3), four parental single-flowering lines consisting of Neelam, Malgoa, Bangalora and Banganapalli and three local single-flowering types (HL 4, HL 6 and HL 12) were selected for intensive screening. To identify the influence of genes in flowering and molecular markers linked with the gene, variation at the DNA level was analysed. A set of 11 primers, OPC2, OPF2, OPF4, OPF7, OPF8, OPQ1, OPX4, OPX6, OPX9, OPX12 and OPX14 producing polymorphic reproducible banding pattern were identified. Amplification reactions with the 11 primers generated 66 bands, 41 of which were polymorphic with size range of 200 bp-3 kb. Results from the dendrogram based on similarity coefficient clearly showed two major groups, X and Y, with a similarity coefficient of 0.4. Cluster X was subdivided into X1 and X2 at a similarity coefficient of 0.56 comprising three selected OP clones and three cultivars (Neelam, Banganapalli and Bangalora) at X1 and one OP clone and one cultivar (Malgoa) at X2. The X1 group included two distinct groups (at similarity coefficient of 0.66) X1.1 and X1.2. Cluster X1.1 was subdivided into X1.1.1 and X1.1.2 at a similarity coefficient of 0.68. Cluster X1.1.1 comprises two multiflowering clones GL 1 and GL 2, with a similarity of 80% between them, closely associated with their morphologically identified maternal parent, Neelam (single-flowering) at the similarity index of 74%. Cluster X1.1.2 comprising clone GL 3 was related to Neelam and its OP clones at the similarity of 68% and with the cluster comprising Banganapalli and Bangalora at the similarity index of 66%. Cluster Y comprises two collections (HL 6 and HL 12) of single-flowering type. Though these clones resembled Neelam morphologically, only 40% similarity was exhibited at the molecular level.