Analysis on the genetic relationship of some mango (Mangifera indica L.) germplasms by ISSR markers.
Wang JiaBao, Wang LingXia, Du ZhongJun, Lei XinTao, Chen YeYuan, Xu BiYu
Author Affiliation: Institute of Tropical Biotechnology, State Key Laboratory of Biotechnology for Tropical Crops, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
Acta Horticulturae Sinica 34 : 87-92
Abstract : The genetic relationships of 38 mango (Mangifera indica L.) accessions were examined by ISSR markers. Of the 60 ISSR primers screened, only 10 primers gave reproducible, polymorphic DNA amplification patterns with a total 79 of amplified bands. Their percentage of polymorphic band was 86.7%. By banding patterns obtained from these 10 primers, each accession could be distinguished from the others except either Hongjinlong or Guifei, since the two accessions had the same amplified band patterns. Genetic distances among the tested accessions ranged from 0 to 0.682 with an average of 0.393. Five groups were formed by UPGMA clustering and the classification results were discussed in this paper.