Identification of closely related mango cultivars by ISSR.
He XinHua, Li YangRui, Guo YongZe, Ou ShiJin, Li RongBai
Author Affiliation: Laboratory of Guangxi Crop Genetic Improvement and Biotechnology, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Guangxi Zhiwu / Guihaia 27 : 44-47
Abstract : Seven carabao mango cultivars or lines and Liuzhou lusong mango (Mangifera indica) were examined with ISSR primers. Of the 30 primers screened, 6 primers gave reproducible, polymorphic DNA amplification patterns, and were selected to construct a DNA fingerprinting map to distinguished carabao mango cultivars or lines. According to the banding patterns, all cultivars tested in this study were distinguished from each other by every one of 6 selected primers and showed ample genetic diversity, indicating that ISSR-PCR was an effective method for cultivar identification of mango cultivars and lines. Based on UPGMA analysis of 69 selected bands, the carabao showed the lowest similarity to all other cultivars while Gaozhou carabao, Zhanjiang carabao, Tianyang xiangmang, Jinqianmang, Liuzhou lusong, Yueximang No. 1 and Panxi red carabao could be clustered into one group.