Genetic diversity of wild Mangifera indica populations detected by ISSR.
Yu XianMei, Ai ChengXiang
Author Affiliation: Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Science, Danzhou, Hainan 571737, China.
Journal of Fruit Science 24 : 329-333
Abstract : The genetic diversity of 265 individuals from 12 populations in Hainan, Yunnan and Guangxi was investigated by using inter-simple sequence repeat (ISSR) markers. 102 bands were amplified by 9 informative and reliable primers, of which 89 were polymorphic loci. A relatively high level of genetic diversity was revealed: PPL=87.25, He=0.254 1, H0=0.394 0 (at species level); PPL=66.81, He=0.196 8, H0=0.309 9 (at population level). A higher level of genetic differentiation was detected among populations with Nei's GST analysis and analysis of molecular variance (AMOVA; GST=0.233 7, FST=0.219 2). Some differentiation may result from habitat fragmentation and gene flow. UPGMA dendrogram shows that populations from Napo, Yongning, Pingnan (all of Guangxi) and Wenshan (of Yunnan) grouped together, whereas Yongde and Banna (of Yunnan) clustered in an isolated clade. The results showed that a mixed mating system was possibly the main factor influencing the genetic structure of this species. These results, combined with other information about M. indica, may provide a valuable basis for proposing conservation strategies.