Analysis of genetic relationships among mango germplasm using ISSR marker.
Xie J. H., Ma W. H., Liu C. M.
Author Affiliation: Southern Subtropical Crop Research Institute, China Tropic Agriculture, Academy of Science, Zhanjiang 524091, China.
: 185-190
Abstract : Thirty-two mango cultivars (Mangifera indica L.), including 13 Chinese, 9 USA, 4 Indian, 2 Australian, 2 Indonesian, 1 Thai and 1 Philippine cultivars, were examined using simple sequence repeat (SSRs) anchored primers. 15 primers gave reproducible DNA amplification patterns, and revealed a total number of 245 DNA bands, in which 215 bands were polymorphic; the percentage of polymorphic loci was 87.7%. The number of generated bands ranged from 6 to 26 per primer. According to the band patterns obtained from these primers, all the cultivars could be distinguished from each other. NTSY-2.0 software was used to calculate the Dice similarity coefficiency, and a dendrogram was constructed based on UPGMA cluster analysis. The mango cultivars were divided into 5 groups. The Indonesian cultivar 'Arumanis' was very far in genetic distance from the others and fell into a group by itself. Nine USA and 2 Chinese cultivars with red skin were placed in a single group. Nine Chinese, 2 Australian, 1 Thai and 1 Philippine cultivars belonged to a distinct group. The others, including 4 Indian and 2 Chinese cultivars were scattered into two groups. On the basis of these results and information on geographic origin, embryo-type, skin color and family structure, mango cultivars could be divided into five classes: (I) Red skin type, generally originating from America or west, (II) Indo-Chinese type, coming from southeast Asia, (III) Southern Indian mango type, (IV) Northern Indian mango type, and (V) Indonesia type.