Role of thidiazuron in in vitro induction of embryogenesis in nucellar tissue of Mangifera indica L. var. Dashehari, leading to plantlets.
Kidwai N. R., Jain M. B., Chaturvedi H. C.
Author Affiliation: Amity University, Lucknow 226 010, India.
Current Science 96 : 1119-1124
Abstract : High frequency differentiation of embryos took place in 100% explants of predisposed nucellar tissue of Mangifera indica L. var. Dashehari, proliferated using 2,4-dichlorophenoxyacetic acid for a short duration of only 7 days initially, particularly under the influence of thidiazuron. The nucellus contained in ovular halves of 25- to 30-day-old young fruits proliferated into dark-brown compact tissue, over which differentiation of discrete sectors of whitish, pale and granular proembryogenic tissue was induced. Enormous number of embryos differentiated in the proembryogenic tissue during its subsequent subculture in the optimum treatment containing 0.15 mg l-1 each of 6-benzylaminopurine and 2-isopentenyladenine, along with 0.5 mg l-1 indole-3-acetic acid. Amongst the embryos of five developmental stages cultured in the liquid-processing medium, the best development was evinced by embryos with av 1.51 cm length. However, amongst the processed embryos, 90% matured and 80% germinated, while 60% did actually convert into plantlets. The properly acclimatized and in vitro-raised plantlets showed more than 80% transplant success, and during 4 months in the potted pure Soilrite under ex vitro conditions displayed an increase in shoot length and produced new leaves as well.