Phylogenetic relationships and diversity of Guignardia spp. isolated from different hosts on ITS1-5,8S-ITS2 region.
Wickert E., Goes A. de, Lemos E. G. de M., Souza A. de, Silveira É. L. da, Pereira F. D., Rinaldo D.
Author Affiliation: Departamento de Fitossanidade FCAV/UNESP, Jaboticabal-SP, Brazil.
Revista Brasileira de Fruticultura 31 : 360-380
Abstract : Fungi of Guignardia genera are commonly isolated from different plant species and most of the time they are characterized as endophytics. However, some species of this genus, as G. citricarpa and G. psidii are known as causal agents of serious diseases that affect cultures, such as the citrus black spot and the guava fruit rot, respectively. They are also responsible for diseases that cause foliar spots in different fruit species and also in other cultures. Studies were conducted to isolate, identify and characterize the genetic diversity present among Guignardia isolates obtained from citrus, mango, guava, eucalyptus, Brazilian grape tree and Surinam cherry by analysis of DNA sequence from cístron ITS1-5,8S-ITS2. It was verified that the obtained isolates belonged to G. mangiferae and G. citricarpa species. Two different Guignardia types, that were found in mango, were not identified and did not belong to any of the species deposited in the GenBank. It was found that mango, guava, eucalyptus, Brazilian grape tree and Surinam cherry were hosts of only G. mangiferae, whereas citrus was the host G. mangiferae and G. citricarpa species. Mango hosted 3 different Guignardia sp, G. mangiferae and 2 other types that remained without identification at the species level. It was also verified that isolates of Guignardia obtained from guava fruit rot symptoms were identified as G. mangiferae.