Construction of AFLP molecular marking system in Mangifera indica L.
Wang Yuan, Jin ZhiQiang, Chen YeYuan, Lei XinTao
Author Affiliation: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
Agricultural Science & Technology - Hunan 10 : 72-75
Abstract : Objective: The study aimed to construct the AFLP molecular marking system in Mangifera indica. Method: Four varieties of Mangifera indica were used to explore new ways for high-quality DNA, and AFLP analysis of 31 varieties of Mangifera indica was carried out to detect the varietal genetic diversity. Result: 14 pairs of primers with stronger polymorphism, better banding patterns and higher resolution were screened out from 64 pairs of selective amplification primers. Then they were used to analyse the fingerprint of 31 varieties of Mangifera indica, the results showed that the ratio of polymorphic bands amplificated by the 14 pairs of primers reached 97% in 31 varieties of Mangifera. Conclusion: It was suggested that AFLP was suitable for detecting the polymorphism of Mangifera indica resources.