Control of growth and identification of microorganisms contaminants at in vitro propagation of lateral mango buds.
Andrade S. R. M. de, Oliveira W. C. de, Reis Junior F. B. dos, Charchar M. J. d'A., Faleiro F. G., Mehta A., Teixeira J. B., Peixoto J. R.
Author Affiliation: Embrapa Cerrados, BR 020, Km 18, Rod. Brasília/Fortaleza, Caixa Postal 08223, CEP 73310-970 Planaltina, DF, Brazil.
Boletim de Pesquisa e Desenvolvimento - Embrapa Cerrados : 27 pp.
Abstract : The objective was to improve the protocols for surface decontamination, and identify the fungi and bacteria, and control bacteria and fungi growth. We identify 9 genera of fungi, and found that the contamination by fungi came from external fragments of tissue. We tested the effect of the following fungicides at the experimental field: 20 gL-1 Tebuconazole, 20 gL-1 of Mancozeb, 20 gL-1 Procymidone. There was no significant difference between treatments. The in vitro effect of the fungicides were tested. The active principle Tebuconazole showed higher efficiency in the control of fungi on the surface of the explant. Evaluations of the bacteria, called B, demonstrated that it is Gram negative, has flat and circular colonies of white color. Analysis by partial sequencing of 16S rRNA gene identified that it could be phylogenetically close to the genera and Asaia and Swaminathania. The extract of propolis was effective in controlling the growth of bacteria at concentrations 0.5 and 1% in contrast to the crude propolis which did not control the growth of the bacteria.