Effect of low temperature on nucleolus and chromosomes' behavior of Mango (Mangifera indica L.) PMC during meiosis.
Huang JingHao, Wu HongXia, Wang SongBiao, Liang GuoLu, Wang WeiXing, Ma WeiHong
Author Affiliation: South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong 524091, China.
Acta Horticulturae Sinica 35 : 1101-1108
Abstract : The developed smearing method was adopted to observe the process of PMC meiosis of Mango (Mangifera indica L.) 'Tainong 1' and 'Irwin' in this article. The results were as follows: The cytoplasm devision of mango PMC was simultaneous type. Under normal temperature (25-30°C), the process of pollen mother cell meiosis is normal; The most of chromosomes become bivalents which like V, O, Y, X, sticks and dots, and mainly like dots, V and O, but a few of univalent (0.79% of Tainong 1 and 0 of Irwin) and multivalent (4.76% of Tainong 1 and 3.57% of Irwin) had been observed. While under natural low temperature (10-20°C), a great deal of abnormalities, including micro nucleoli, scattered and lagged chromosomes and anaphase bridges were detected during PMC meiosis, even though the abnormalities were different between the two cultivars; The ratio of micro nucleoli was 22.88% of average, which was obviously higher than that of control (4.56% of average under normal temperature); And the ratios of univalent and multivalent in diplotene, which increased seriously, were 8.82% and 17.65% of Tainong 1, and 14.52% and 24.19% of Irwin under natural low temperatures. Abnormalities during meiosis under natural low temperatures might play a role in male sterile.