Cloning and bioinformatic analysis of the AP1 homolog gene from mango.
Luo Cong, He XinHua, Chen Hu, Jiang YaQin, Gao MeiPing, Li YangRui
Author Affiliation: College of Agriculture, Guangxi University, Nanning, 530004, China.
Genomics and Applied Biology 28 : 851-858
Abstract : In this paper, we cloned two full-length cDNA sequences of homologous gene AP1 by employing RT-PCR, which named as MAP1-1 and MAP1-2. The accession number at GenBank are FJ529206 and FJ529207, respectively. The MAP1-1 contains an 741 bp open reading frame (ORF) corresponding to a deduced protein of 248 amino acids, while the estimated molecular weight and isoelectric point of the putative protein were 28.54 kD and 8.31. The MAP1-2 with a open reading frame (ORF) of 744 bp, encoding 248 amino acid with a predicted molecular mass of 28.78 kD and a pI of 8.70. A comparison of the deduced amino acid residues indicated that their nucleotide sequences have a range of 72% to 81% identities with AP1 gene homologues of other woody plants. The experimental results indicated that MAP1-1 and MAP1-2 had MADS-box region between the 1st and 61th amino acid and K-box region between the 88th and 178th amino acid. Both genes were located in the nucleus, however, the distribution of functional positions was different between these two genes which may cause different behavior during the growth of floral organ. Prediction of the secondary structure of the protein showed that MAP1-1 protein had 12 ? helix, 4 ? sheet and 14 ? turn, while MAP1-2 protein had 11 ? helix, 5 ? sheet and 15 ? turn. Most of the amino acids were observed to be hydrophilic. The research was beneficial to the further understanding of the molecular mechanism of flowering and biological developmental stages of flowering.