Screening of various Trichoderma isolates collected from Pakistan with their subsequent genetic homology by RAPD analysis.
Amna Ali, Rukhsana Bajwa, Ghazala Nasim, Nasir Mehmood, Rasheda Jabeen
Author Affiliation: Institute of Plant Pathology, University of the Punjab, Lahore, 54590, Pakistan.
International Journal of Biology and Biotechnology 7 : 383-391
Abstract : A total of 23 isolates of Trichoderma spp. collected from different areas of Pakistan, were procured from first Fungal Culture Bank of Pakistan (PCBP), Institute of Plant Pathology, University of the Punjab, Lahore. On the basis of morphological and cultural characteristics, Trichoderma isolates were identified into four species, namely T. harzianum, T. hamatum, T. koningii and T. pseudokoningii. The genomic homology of these isolates of Trichoderma was examined using RAPD analysis. Out of 27 random decamer primers, five primers viz., A-11, A-12, A13, B-05 and B-18 were screened out at 25°C optimized Tm (melting temperature). The amplification of bands were observed in the range of 250 bp-50000 bp by all five primers. In cluster analysis of five primers, isolate of T. koningii (wood of M. indica) showed least genomic similarity of 47.30% in comparison to all others. The other isolates of T. harzianum, T. hamatum, T. koningii and T. pseudokoningii showed maximum genomic homology (~78%). This data will be useful to achieve authentic identification for maximum usage of these microbes in all research fields.