Molecular analysis in the differentiation of Colletotrichum gloeosporioides isolates from the cashew and mango trees.
Serra I. M. R. de S., Menezes M., Coelho R. S. B., Ferraz G. M. G., Montarroyos A. V. V., Martins L. S. S.
Author Affiliation: Fitossanidade, Departamento de Agronomia, Universidade Federal Rural de Pernambuco, R. Dom Manuel de Medeiros, S/N, 52171-900, Recife, PE, Brazil.
Brazilian Archives of Biology and Technology 54 : 1099-1108
Abstract : The aim of the present work was to analyze the molecular methods in the differentiation of Colletotrichum gloeosporioides isolates obtained from the cashew and mango trees. The different molecular taxonomic methods used proved to be efficient regarding intraspecific characterization. Similarly, molecular methods also proved to be efficient in differentiation of the C. gloeosporioides isolates in relation to host specificity. In the analysis of the ITS sequence of the ribosomal DNA, all the isolates amplified with the CgInt and ITS4 primers, confirming that they pertained to C. gloeosporioides. The results from this study suggested that methods based on the pathogenicity, isozyme analysis and RAPD were effective in differentiating C. gloeosporioides isolates from the cashew and mango trees.