Pathogenicity of cell wall degrading enzymes produced by Botryodiplodia theobromae against mango fruit.
Li Min, Gao ZhaoYin, Hu MeiJiao, Zhou Shuo, Yang DongPing, Yang Bo, Yi JianXue, Yang FengZhen
Author Affiliation: Environment and Plant Protection Research Institute, CATAS, Danzhou, Hainan 571737, China.
Journal of Fruit Science 28 : 1054-1058
Abstract : To confirm the effects of cell wall degrading enzymes (CWDEs) produced by Botryodiplodia theobromae Pat. on mango fruits, the activity of 5 CWDEs were examined, and the pathogenicity and the ability of producing CWDEs of four fungal pathogens causing mango stem rot, such as Colletotrichum gloeosporioides Penz., Phomopsis mangiferae Ahmad and Dothiorella dominicana Pet.et Cif. were studied. B. theobromae could produce polygalacturonase (PG), pectinmethylgalacturonase (PMG), polygalacturonic acid trans-eliminase (PGTE), pectin methyltrans-eliminase (PMTE) and cellulase (Cx.) when cultured in vitro or in vivo. The activity of PG, Cx and PMG were significantly higher than that of PGTE and PMTE. Among three primary CWDEs, the activity peak of PG and Cx appeared earlier and PMG's peak came late. The pathogenicity of B. theobromae was significantly stronger than that of other three pathogens, and the ability of producing pectinase of B. theobromae was remarkably stronger than that of other three pathogens.