Changes in the level of growth hormones during fruit bud differentiation and identifying the morphological and histological differences of healthy and malformed shoots and buds of mango.
Palanichamy V., Bhaskar Mitra, Aranganathan
Author Affiliation: School of Biosciences and Technology, VIT University, Tamil Nadu, India.
International Journal of Pharmacy and Technology 3 : 3569-3579
Abstract : The present study was carried out to find out the changes in the level of phytohormones in three mango hybrids namely Amrapali, Pusa Arunima and Mallika during the period from last week of August to the last week of December in 2003-04 and 2004-05 and the study was also carried out to find out the morphological and histological differences between the malformed shoots and or buds of Amrapali and Bhadauran. Changes in the quantity of phytohormone during the study period was analyzed by HPLC. To identify the differences between healthy and malformed shoot and or bud, dissected buds and or shoots were observed under microscope. From the first part of the study it was found that the Indole Acetic Acid and the Abscisic acid have been found to be increasing and the trend was opposite in case of Gibberellic acid like substances during the period of fruit bud differentiation i.e. from 15th October to 15th December in all the hybrids during the study period. From the second part of the study, morphologically healthy inflorescence axis has been found long, slender having normal light green color and normal buds whereas the malformed inflorescence was thicker, short having dark green color with larger buds in comparison to the healthy ones. Histologically the diameter of the main axis of malformed inflorescence of the Amrapali was found bigger than the healthy inflorescence. The area occupied by various tissues like cortex, vascular bundles and pith in the main axis of inflorescence appeared thicker in malformed inflorescence of Amrapali in comparison to the healthy inflorescence of Bhadauran. The healthy buds had been found symmetrical whereas, the main axis of malformed buds had asymmetrical shape. The malformed buds were appeared generally distorted and leaf primordia were not clearly visible as against the healthy buds of Bhadauran.