Application of PCR-RFLP in detecting benzimidazoles-resistant isolates of Colletotrichum gloeosporioides from mango in Tainan area.
Lou P. S., Huang Y. J., Chung W. C., Cheng A. S., Chung W. H.
Author Affiliation: Department of Plant Pathology, National Chung Hsing University, Taichung, Taiwan.
Plant Pathology Bulletin 19 : 255-260
Abstract : For examining the sensitivity of PCR-RFLP developed for testing benzimidazole-resistant Colletotrichum gloeosporioides, a total 192 isolates of C. gloeosporioides obtained from mango producing area, Zuojhen (7 isolates), Danei (21 isolates), Shanshang (6 isolates), Yujing (71 isolates), Guantian (32 isolates), Nanhua (46 isolates) and Nansi (9 isolates), in Tainan. The results showed that the partial ?-tubulin gene of all C. gloeosporioides isolates could be amplified by primers TubGF1 and TubGR. Consequently, 158 PCR products could digest into two bands by restriction enzyme of Bsh 1236I. Furthermore, the EC50 (mg a.i./l) of three benzimidazoles of benomyl, carbendazim and thiabendazole to the isolates of PCR product digested are 128-467, 111-415 and 146-433 mg a.i./l. According to the results, the isolates of C. gloeosporioides from mango in Tainan are high risk population to benzimidazoles.