Assessment of genetic diversity among different indigenous Xanthomonas isolates via randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR).
Sabin Fatima, Rukhsana Bajwa, Tehmina Anjum, Zafar Saleem
Author Affiliation: Institute of Agriculture Sciences, Quaid-e-Azam Campus, University of the Punjab, Lahore, Pakistan.
African Journal of Microbiology Research 6 : 1947-1957
Abstract : The genetic diversity among seven Xanthomonas isolates representing four species was assessed using randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) PCR-based techniques. Both techniques revealed high degrees of polymorphisms among the studied isolates. Cluster dendrogram based on combined data of RAPD and ISSR showed that genetic diversity exists in local isolates of Xanthomonas. In terms of percentage similarity values, the genomic variation was found to be in the range of 29.29 to 100% among the isolates. X. campestris (Mangifera indica) remain unclustered in cluster dendrogram and showed a unique genomic profile as compared to other isolates used in this study.