Damage caused by enhanced UV-B radiation to chromosomes in leaves of mango in vitro.
Liu Peng, Xu ChiMo, Luo Wei, Zhou KaiBing
Author Affiliation: Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources, Hainan University, Haikou 570228, Hainan, China.
Plant Physiology Communications 48 : 161-165
Abstract : The leaves of mango (Mangifera indica cv. Tainong No.1) in vitro were used to study chromosome damage after enhanced UV-B radiation, using a single cell gel electrophoresis assay. Results showed that the young leaves treated with 4 hours of UV-B radiation, and the adult and old leaves treated with 6 hours of UV-B radiation began to reveal comet tails of chromosomes. Meanwhile, the OTM (olive tail moment) values of all the leaves rose quickly. From then on, the length and density of comet tails and the OTM values of all leaves rose constantly. The leaves under natural light had no obvious comet tail; their OTM values were lower and had no obvious change. Under UV-B radiation, the comet tails of the young leaves were most obvious and their OTM values were highest, and those of adult leaves were second highest.