Isolation and characterization of Nitraria sibirica actin gene.
Wang Li, Li FeiFeng, Zhang WenBo, Chen GuiLin, Lin XiaoFei
Author Affiliation: College of Life Sciences, Inner Mongolia University, Hohhot 010021, China.
Acta Prataculturae Sinica 21 : 151-158
Abstract : Nitraria sibirica actin gene (NsAct) was cloned using degenerated-PCR and RACE technique, and gene structure and expression pattern were analyzed. Full-length sequence of the NsAct cDNA contains 1 831 bp, which has a 1 134 bp open reading frame (ORF) encoding 377 amino acids. Genomic DNA of the NsAct contains 2 913 bp, including 5 extrons and 4 introns. The NsAct amino acid sequence shows a high homology to that of the other plants, and phylogenetic tree generated from the nucleotide sequences of plant actins revealed that the NsAct shows close genetic relation with Mangifera indica (sapindales). The NsAct equally expressed in root, stem, and leaf, and no obvious change in expression level was observed under stress conditions of drought, salt, cold, and exogenous abscisic acid (ABA).