Determination of paclobutrazol residue in mango fruit using a matrix solid-phase dispersion method coupled to high-performance liquid chromatography with ultraviolet detection.
Parvathamma T., Ramesh A., Rao T. N.
Author Affiliation: Department of Analytical Chemistry, International Inisitituate of Biotechnology and Toxicology (IIBAT), Padappai, Chennai - 601 301, TamilNadu, India.
International Journal of ChemTech Research 4 : 1473-1477
Abstract : A simple, sensitive and inexpensive method was developed using matrix solid-phase dispersion (MSPD), together with high performance liquid chromatographic method for determination of paclobutrazol in mango fruite. The evaluated parameters included the type and amount of sorbent (silica gel and Alumina) and the nature of eluent (Tetrahydrofuron, Acetonitrile and MilliQ water). The best results were obtained using 1.0 g of mango fruit sample, (1.0 g of silica gel as sorbet and 20 ml of Tetrahydrofuron - Acetonitrile - MilliQ water (1:1:1), (v/v)). The method was validated using mango fruit samples spiked with paclobutrazol at different concentration levels (0.03 and 0.3 µg/mL). Average recoveries (using each concentration six replicates) ranged 89-93%, with relative standard deviations less than 3%, calibration solutions concentration in the range 0.01-2.0 µg/mL and limit of detection (LOD) and limit of quantification (LOQ) were 0.01 µg/mL and 0.03 µg/mL respectively.