Ethanolic extract of Mangifera indica seed kernel ameliorates visceral fat via improvement in lipid metabolism in high fat diet induced obese mice.
Kankanampati Krishnaveni, Rosaline Mishra, Pushkala K., Velayudam D., Suman Kapur
Author Affiliation: BITS-Pilani, Hyderabad Campus, Jawahar Nagar, Shameer Pet, Hyderabad - 500 078, RR District, Andhra Pradesh, India.
Journal of Pharmacy Research 5 : 4974-4978
Abstract : Aim: Present study was aimed at evaluating the effect of ethanolic extract of Mangifera indica seed kernel (MESK) on induction of obesity and other symptoms associated with visceral obesity syndrome in High-Fat Diet induced obese mice. Methods: HFD fed obese (DIO) Swiss-albino mice (N=6/group) were treated with a single oral dose of 0.75 µg/kg/day of dried MESK or 120 µg/kg/day of Orlistat for a 6 weeks period. Mangifera indica Seed kernels were extracted using Ethanol: Water as solvent and the crude extract was subjected to several phytochemical screenings. Results: Oral treatment with MESK significantly altered the % body weight change, as judged by BMI and LI, WAT (p0.0001) and dyslipidemia as indicated by lowering of serum cholesterol (TC) and triglycerides (TG) in DIO mice, as compared to untreated DIO mice and normal diet fed mice. MESK treatment was also found to improve FPG levels and increase fecal lipid excretion which was comparable to that observed in mice treated with Orlistat. Preliminary phytochemical screening revealed presence of neutral lipid (tri-, di- and monoacylglycerols), and free fatty acids and steroids in the hexane fraction and presence of polar lipid along with several non-lipid substances in the ethyl acetate fraction. The extract also tested positive for presence of saponins, phenolics, flavonoids, steroids and reducing sugars while it showed complete absence of anthroquinones. Conclusion: Our findings suggest that MESK can counter the effect of environmental factors such as high fat diet intake and has a potential to be used as an anti-obesity dietary supplement with desirable glucose and lipid metabolism modulating properties.