Vegetative compatibility of Fusarium mangiferae strains from mango malformation disease.
Liu Feng, Wei JiGuang, Zhan RuLin, Chang JinMei
Author Affiliation: Agricultural College, Guangxi University, Nanning, Guangxi 530004, China.
Journal of Fruit Science 29 : 1092-1096
Abstract : Objective: In order to explicit the vegetative compatibility groups (VGC) of Fusarium mangiferae. Method: The nitrate-nonutilizing (nit) mutant complementation technique was used to explore the population structure of the fungus. Result: The 38 strains of F. mangiferae were isolated from different mango cultivars from Panzhihua, Sichuan and Huaping, Yunan Providence in China. The tested single-conidium strains were then transplanted on KClO3-containing potato sucrose agar (KPS) plates to mutagenize nit mutants and 455 nit mutants were recovered from the isolates by transferring chlorate-resistant sectors from KPS plates to minimal medium (MM). The four mutant types were identified as nit A, nit B, nit C and nit D based on the growing situation of the mutants on three kinds of different nitrogen media, MM, nitrite medium (NM) and hypoxanthine medium (HM). Among the four types, nit A was the most common one, with the frequency of 78.02%. Next were nit B and nit C, with the frequency of 7.91% and 13.63% respectively. The least one was nit D, 0.44%. The nit mutants then were paired on MM plates to test the vegetative compatibility of all isolates, and totally five different VCGs were identified. 38 strains were assigned to 5 VCG, 30 strains in VCG1, 3 strains in VCG2, VCG3 and VCG4 contains 2 strains, respectively, strain MG33 formed separate VCG5. Conclusion: The correlation between vegetative compatibility of F. mangiferae and mango cultivars or geographical origin was insignificant.