Assessment of genetic diversity among different indigenous Xanthomonas isolates via RAPD and ISSR.
Sabin Fatima, Rukhsana Bajwa, Tehmina Anjum, Zafar Saleem
Author Affiliation: Institute of Plant Pathology, Quaid-e-Azam Campus, University of the Punjab, Lahore, Pakistan.
Archives of Biological Sciences 64 : 307-319
Abstract : The genetic diversity among seven Xanthomonas isolates representing four species was assessed using RAPD and ISSR PCR-based techniques. Both techniques revealed high degrees of polymorphisms among the studied isolates. A cluster dendrogram based on the combined data of RAPD and ISSR showed that genetic diversity exists in local isolates of Xanthomonas. In terms of percentage similarity values, the genomic variation was found to be in the range of 29.29%-100% among the isolates. X. campestris (Mangifera indica) remained unclustered in cluster dendrogram and revealed a unique genomic profile compared to other isolates used in this study.