Pathogenicity of cell wall degrading enzymes produced by Botryodiplodia theobromae Pat. against mangoes.
Li Min, Gao ZhaoYin, Hu MeiJiao, Zhou Shuo, Yang DongPing, Yang Bo, Yi JianXue, Yang FengZhen
Author Affiliation: Environment and Plant Protection Research Institute, CATAS, Haikou 571101, China.
Agricultural Biotechnology 1 : 18-21
Abstract : Objective: This study aimed to confirm the roles of cell wall degrading enzymes (CWDEs) produced by Botryodiplodia theobromae Pat. in the infection of mango fruits. Method: Change of activities of five types of CWDEs produced by B. theobromae Pat. were studied under both in vitro culture and inoculation conditions, along with the pathogenicity and the ability of producing CWDEs of four post-harvest fungal pathogens (B. theobromae Pat., Colletotrichum gloeosporioides Penz., Phomopsis mangiferae Ahmad and Dothiorella dominicana Pet. et Cif.) which cause stem-end rot of mangoes. Result: B. theobromae Pat. was able to produce polygalacturonase (PG), pectinmethylgalacturonase (PMG), polygalacturonic acid trans-eliminase (PGTE), pectin methyltrans-eliminase (PMTE) and cellulase (Cx.) under both in vitro culture and inoculation conditions, of which activities of PG, Cx and PMG were significantly higher in than that in either PGTE or PMTE. Among three primary CWDEs, the peak of activities of PG and Cx appeared earlier and that of PMG occured later. The pathogenicity of B. theobromae Pat. was significantly higher than that of any other three pathogens; it is the same with the abilities of producing pectinase. Conclusion: This paper provides theoretical bases for further exploring the mechanism of host-pathogen interaction and decreasing the post-harvest loss of mango fruits.