Ogataea kanchanaburiensis sp. nov. and Ogataea wangdongensis sp. nov., two novel methylotrophic yeast species from phylloplane in Thailand.
Limtong S., Kaewwichian R., Groenewald M.
Author Affiliation: Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
Antonie van Leeuwenhoek 103 : 551-558
Abstract : Three strains (KM03T, KM13T and KM15) representing two novel methylotrophic yeast species were isolated from the external surface of plant leaves, which were collected from Kanchanaburi province, Thailand, by three-consecutive enrichments in methanol broth. Strain KM03T was isolated from phylloplane of a mango tree (Mangifera indica) and two strains, KM13T and KM15, were obtained from phylloplane of different wine grapes (Vitis vinifera). The sequences of the D1/D2 region of the large subunit (LSU) rRNA gene of the two strains (KM13T and KM15) were identical and differed markedly from that of strain KM03T. In terms of pairwise sequence similarity of the D1/D2 region the closest species to the strains KM13T and KM15 were Candida suzukii (CBS 9253T) and Candida nitratophila (CBS 2027T) but with 2.1% nucleotide substitutions. Strain KM03T differed from Ogataea wickerhamii (CBS 4307T), its closest relative, by 2.3% nucleotide substitutions. Phylogenetic analysis based on the D1/D2 sequences placed the three strains in the Ogataea clade. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, the sequence analyses of the D1/D2 and the internal transcribed spacer (ITS) regions of the nuclear ribosomal RNA gene (nrRNA) operon, the three strains represent two novel Ogataea species although formation of ascospores was not observed. Ogataea kanchanaburiensis sp. nov. is proposed for strain KM03T (=BCC 47626T=NBRC 108603T=CBS 12673T). Two strains, KM13T and KM15, were assigned to Ogataea wangdongensis sp. nov. (type strain KM13T=BCC 42664T=NBRC 107778T=CBS 12674T). GenBank/EMBL/DDBJ accession numbers for the sequences of the D1/D2 and the ITS regions of O. kanchanaburiensis KM03T are AB734090 and AB734093, respectively, of O. wangdongensis KM13T are AB734091 and AB734094, respectively, and of O. wangdongensis KM15 are AB734092 and AB734095, respectively.