Purification and bio-control mechanism against mango anthracnoses of antifungal protein of mangrove endophytic bacteria AiL3.
Yu Sha, He Hong, Zhan RuLin, Ma LingYan, Li Lin
Author Affiliation: College of Agriculture, Guangdong Ocean University, Zhanjiang 524088, China.
Chinese Journal of Biological Control 29 : 104-109
Abstract : An antifungal protein was separated and purified from endophytic bacterium AiL3 in mangrove Acanthus ilicifolius. The efficacy on mango anthracnose and bio-control mechanism was investigated. Results showed that the antifungal protein had strong antagonistic activity to Colletotrichum gloeosporioides. The molecular weight of antifungal protein from strain AiL3 was 28 kD. Control efficiency achieved 45.1% at 9 days after the antifungal protein treatment of concentration 60 µg.mL-1, which had no significant difference with that of Sportak (43.6%). The antifungal protein could inhibit the hyphal growth and caused abnormal hyphal morphology of C. gloeosporioides, such as swollen, curliness and deformity. The treatment could also disrupt the structure of cell membrane, thereby induced the leakage of cytoplasm. The activities of peroxidase (POD) and phenylalanine ammonia-lyase (PAL) of mango after the treatment were decreased and increased, respectively.