Molecular identification and characterization of Colletotrichum gloeosporioides causing anthracnose disease in mango (Mangifera indica L.).
Mishra R. K., Ashutosh Pandey, Mathew A. J., Pandey B. K.
Author Affiliation: Molecular Plant Pathology Laboratory, Central Institute for Subtropical Horticulture, Lucknow - 227 107, India.
Biochemical and Cellular Archives 13 : 97-103
Abstract : Anthracnose, a serious pre and post-harvest disease of mango is known to be caused by Colletotrichum gloeosporioides Penz. and Sacc. Twenty five isolates of C. gloeosporioides were isolated from mango anthracnose samples collected from different agro-climatic zones of India. Primers Cg1NT and ITS4 had confirmed the identity of the isolates of the C. gloeosporioides identified by traditional PCR amplification. These isolates were evaluated for their pathogenic variability on mango seedlings and also genetic variability using random amplified polymorphic DNA (RAPD) markers. Out of twelve RAPD primers screened, five primers, viz., OPA-1, 4, 5, 9 and 12 were able to produce more reproducible banding pattern. Each of these primers generated a spectrum of scorable amplicons in a range of 500 to 2300 bp. A total of 113 amplified bands were scored with average of 5.58 no. of polymorphic bands. Cluster analysis revealed three major clusters and one minor cluster. Major clusters comprised the isolates according to their geographical adaptations but some mix up from different regions were found in the minor cluster. These results suggest that there is maximum genetic similarity between isolates within the similar agro-climatic regions and variation between different agro-climatic regions. However, clustering pattern did not follow the cultural and morphological characters.